Positive-selection vector for direct protein expression
نویسندگان
چکیده
منابع مشابه
Positive-selection vector for direct protein expression.
We describe the development of a novel positive-selection vector, RHP-AmpS, that is suitable for seamless cloning and high-level protein expression in Escherichia coli. In this vector, beta-lactamase (Bla) was rendered nonfunctional by replacing the codon for the C-terminal amino acid of the beta-lactamase gene (bla) with a stop codon. Insertion of a target gene in the correct orientation (tail...
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fied tubulins, p. 478-485. In R.B. Vallee (Ed.), Methods in Enzymology, Vol. 196. Academic Press, New York. 3.Nogales, E., S.G. Wolf and K.H. Downing. 1998. Structure of the αβ tubulin dimer by electron crystallography. Nature 391:199-203. 4.Schiff, P.B., J. Fant and S.B. Horwitz. 1979. Promotion of microtubule assembly in vitro by taxol. Nature 277:665-667. 5.Schiff, P.B. and S.B. Horwitz. 198...
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A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113091